Visium CytAssist FFPE v4 -- University of Minnesota TMCs
Laura J Niedernhofer, David A Bernlohr
Published: 2023-12-21 DOI: 10.17504/protocols.io.n92ldmn99l5b/v4
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Abstract
The Visium CytAssist Spatial Gene Expression for FFPE assay is designed to analyze mRNA in
tissue sections derived from formalin fixed & paraffin embedded (FFPE) tissue samples. The Visium
CytAssist instrument requires a glass slide with intact tissue sections as input.
Tissue sections are processed with Visium Spatial Gene Expression assay performed by the University of Minnesota Imaging Center and sequenced by the University of Minnesota Genomics Center.
Steps
Deparaffinization, H&E Staining, Imaging & Decrosslinking
1.
CG000520_Demonstrated_Protocol_VisiumCytAssist_Deparaffin_H_E_RevB.pdf
FASTQ Generation
3.
BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq software version 2.20.0
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