Precise genome engineering inPseudomonasusing phage-encoded homologous recombination and the Cascade–Cas3 system

Xue Wang, Wentao Zheng, Yandong Xia, Shiqing Gao, Diao Zhou, Vinothkannan Ravichandran, Chanjuan Jiang, Qiang Tu, Yulong Yin, Youming Zhang, Jun Fu, Ruijuan Li, Jia Yin

Published: 2023-08-24 DOI: 10.1038/s41596-023-00856-1

Pseudomonas genome engineering

Phage-encoded homologous recombination

Cascade-Cas3 system

Recombineering efficiency

Genetic manipulation

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Sections

Figures

References

Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Determination of the resistance and transformation characteristics of Pseudomonas
- Two-step seamless modification and three-step seamless modification
Acknowledgements

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Precise genome engineering inPseudomonasusing phage-encoded homologous recombination and the Cascade–Cas3 system

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